[Identification of influenza virus type A by solid-phase radioimmunological analysis].
Identifieur interne : 000214 ( 1957/Analysis ); précédent : 000213; suivant : 000215[Identification of influenza virus type A by solid-phase radioimmunological analysis].
Auteurs : T V Ignat'Eva ; G N Trushinskaia ; E I IsaevaSource :
- Voprosy virusologii [ 0507-4088 ]
Descripteurs français
- KwdFr :
- Animaux, Culture virale, Dosage radioimmunologique (), Embryon de poulet, Hémagglutinines virales (analyse), Immunoglobuline G (analyse), Lapins, Sérums immuns (isolement et purification), Tests d'inhibition de l'hémagglutination, Virus de la grippe A (immunologie), Virus de la grippe A (isolement et purification), Études d'évaluation comme sujet.
- MESH :
- analyse : Hémagglutinines virales, Immunoglobuline G.
- immunologie : Virus de la grippe A.
- isolement et purification : Sérums immuns, Virus de la grippe A.
- Animaux, Culture virale, Dosage radioimmunologique, Embryon de poulet, Lapins, Tests d'inhibition de l'hémagglutination, Études d'évaluation comme sujet.
English descriptors
- KwdEn :
- Animals, Chick Embryo, Evaluation Studies as Topic, Hemagglutination Inhibition Tests, Hemagglutinins, Viral (analysis), Immune Sera (isolation & purification), Immunoglobulin G (analysis), Influenza A virus (immunology), Influenza A virus (isolation & purification), Rabbits, Radioimmunoassay (methods), Virus Cultivation.
- MESH :
- chemical , analysis : Hemagglutinins, Viral, Immunoglobulin G.
- chemical , isolation & purification : Immune Sera.
- immunology : Influenza A virus.
- isolation & purification : Influenza A virus.
- methods : Radioimmunoassay.
- Animals, Chick Embryo, Evaluation Studies as Topic, Hemagglutination Inhibition Tests, Rabbits, Virus Cultivation.
Abstract
High sensitivity and specificity of solid-phase radioimmunoassay (SPRIA) in identification of influenza A (H1N1 and H2N2) viruses in the infected allantoic culture were demonstrated. Mixtures of influenza hyperimmune sera free from antibodies to host cell antigens and antineuraminidase antibodies were used as active test sera. The test serum a-H1N1 consisted of antisera to A/FmI/47, A/Netherlands/36/56, and A/USSR/090/77 strains; it detected practically all tested variants of H1N1 virus isolated in 1947-1982 in allantoic cultures containing virus-specific protein in amounts of 1.4 to 0.7 ng/ml. For the detection of H2N2 subtype viruses (1957-1967), a mixture of antisera to A/Singapore/1/57, A/Leningrad/2/63, A/Gorkiy/62/65, and A/Tokyo/3/67 viruses was used. This test serum could detect H2N2 virus in the allantoic fluid containing 0.7-035 ng/ml of virus protein.
PubMed: 3993004
Affiliations:
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pubmed:3993004Le document en format XML
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V Ignat'Eva</name></author><author><name sortKey="Trushinskaia, G N" sort="Trushinskaia, G N" uniqKey="Trushinskaia G" first="G N" last="Trushinskaia">G N Trushinskaia</name></author><author><name sortKey="Isaeva, E I" sort="Isaeva, E I" uniqKey="Isaeva E" first="E I" last="Isaeva">E I Isaeva</name></author></analytic><series><title level="j">Voprosy virusologii</title><idno type="ISSN">0507-4088</idno></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chick Embryo</term><term>Evaluation Studies as Topic</term><term>Hemagglutination Inhibition Tests</term><term>Hemagglutinins, Viral (analysis)</term><term>Immune Sera (isolation & purification)</term><term>Immunoglobulin G (analysis)</term><term>Influenza A virus (immunology)</term><term>Influenza A virus (isolation & purification)</term><term>Rabbits</term><term>Radioimmunoassay (methods)</term><term>Virus Cultivation</term></keywords><keywords 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comme sujet</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">High sensitivity and specificity of solid-phase radioimmunoassay (SPRIA) in identification of influenza A (H1N1 and H2N2) viruses in the infected allantoic culture were demonstrated. Mixtures of influenza hyperimmune sera free from antibodies to host cell antigens and antineuraminidase antibodies were used as active test sera. The test serum a-H1N1 consisted of antisera to A/FmI/47, A/Netherlands/36/56, and A/USSR/090/77 strains; it detected practically all tested variants of H1N1 virus isolated in 1947-1982 in allantoic cultures containing virus-specific protein in amounts of 1.4 to 0.7 ng/ml. For the detection of H2N2 subtype viruses (1957-1967), a mixture of antisera to A/Singapore/1/57, A/Leningrad/2/63, A/Gorkiy/62/65, and A/Tokyo/3/67 viruses was used. This test serum could detect H2N2 virus in the allantoic fluid containing 0.7-035 ng/ml of virus protein.</div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Ignat Eva, T V" sort="Ignat Eva, T V" uniqKey="Ignat Eva T" first="T V" last="Ignat'Eva">T V Ignat'Eva</name><name sortKey="Isaeva, E I" sort="Isaeva, E I" uniqKey="Isaeva E" first="E I" last="Isaeva">E I Isaeva</name><name sortKey="Trushinskaia, G N" sort="Trushinskaia, G N" uniqKey="Trushinskaia G" first="G N" last="Trushinskaia">G N Trushinskaia</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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